Chemical analysis by laser-based Raman spectroscopy
1 Department of Disease Control, School of Veternary Medicine, University of Zambia, Lusaka , Zambia.
2 Biochemisty Deparment , Lead City University Ibadan,Nigeria.
3 College Of Health Sciences, Obafemi Awolowo University,Ile Ife, Osun State, Nigeria.
Review
International Journal of Scholarly Research and Reviews, 2023, 02(02), 001–004.
Article DOI: 10.56781/ijsrr.2023.2.2.0019
Publication history:
Received on 09 February 2023; revised on 26 March 2023; accepted on 28 March 2023
Abstract:
Raman was first discovered by C.V. Raman and K.F. Krishnan in 1928.It is a spectroscopic technique based on inelastic scattering of monochromatic light usually- from a laser in the visible or near infra-red part of electromagnetic spectrum. It probes vibrational, modes of the molecule and crystals of solid, liquid, and gaseous samples.
t is commonly used in chemical analysis to provide a fingerprint by which molecules can be identified. At the time of its discovery from observing sunlight, obvious challenges were posed by the lack of a good Raman source, lack of a good detector, and by interference from fluorescence, which in some cases overwhelmed the Raman signal. The advent of laser made good for the deficiency of a good Raman source, multichannel detectors like Photodiode Arrays (PDA) or Charge - Coupled Devices (CCD) for a good detector and Fourier –transform for fluorescence.
In addition, instruments such as notch filters, tunable filters, laser stop apertures, double and triple spectrometric systems are now used to reduce Rayleigh scattering and obtain high-quality Raman spectra. Thus, Raman has become the optimal technique for versatile, minimally invasive spectroscopy.
Keywords:
Laser Ray Keyword; Raman Spectroscopy; Keyword Chemometric Analysis; Raman Spectrum; Label Free
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0